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bugbuster protein extraction reagent cat. no. 70584-3  (Millipore)


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    Millipore bugbuster protein extraction reagent cat. no. 70584-3
    Bugbuster Protein Extraction Reagent Cat. No. 70584 3, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bugbuster%C2%AE+protein+extraction+reagent/bugbuster+protein+extraction+reagent/pmc12267110-340-8-12
    Average 90 stars, based on 1 article reviews
    bugbuster protein extraction reagent cat. no. 70584-3 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Lysis:

    Article Title: Identification of functional genes in a chloroform and dichloromethane-degrading microbial culture
    Article Snippet: In a Coy anaerobic glovebox, the pellet was thawed and resuspended in 10 mL anaerobic lysis buffer [50 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.1% Triton-X100, 5% glycerol, 1 mM tris(2-carboxyethyl)phosphine (TCEP), 50 μg/mL leupeptin, 2 μg/mL aprotinin, 10 mM MgCl 2 ], the cells were lysed using BugBuster ® protein extraction reagent (MilliporeSigma), 300 μg/mL lysozyme, and 1 μg/mL DNase.

    Article Title: Method for automated protein purification
    Article Snippet: Other cell lysis methodologies are within the scope of this disclosure, including but not limited to sonication to disrupt cell membranes, chemical lysis (e.g., Bugbuster® Protein Extraction Reagent, Millipore Sigma, Burlington, MA), liquid homogenization (e.g., French Press), mechanical lysis (e.g., rotating blades that grind and disperse cells and tissues), and the like.

    Article Title: Extract2Chip-Bypassing Protein Purification in Drug Discovery Using Surface Plasmon Resonance.
    Article Snippet: The cells were resuspended in BugBuster® Protein Extraction Reagent (Novagen) supplemented with 0.1 mg/mL Lysozyme, 5 U/mL Benzonase, 1 mM PMSF, and PIC without EDTA, then placed on ice for 20 min.

    Article Title: Machine learning reveals signatures of promiscuous microbial amidases for micropollutant biotransformations
    Article Snippet: Induced cells (1 mL volumes) were lysed using the BugBuster® Protein Extraction Reagent (Sigma-Aldrich) according to the manufacturer’s instructions without use of any optional steps.

    Article Title: Supporting Information for Systematic analysis of non-programmed frameshift suppression in E. coli via translational tiling proteomics
    Article Snippet: Cell pellets were resuspended in lysis buffer (BugBuster® Protein Extraction Reagent (MilliporeSigma, MA, USA) supplemented with 1x cOmpleteTM, EDTA-free Protease Inhibitor Cocktail (MilliporeSigma, MA, USA), 1 U/μl rLysozymeTM (MilliporeSigma, MA, USA; final concentration) and 0.5 U/μl Benzonase® Nuclease (MilliporeSigma, MA, USA, final concentration)) resulting in a final OD600 of 20, calculated according to: X μl lysis buffer = OD600 x ml of culture pelleted x 20.

    Article Title: T cell activating bispecific antigen binding molecules
    Article Snippet: The bacterial pellet was resuspended with 25 ml BugBuster® Protein Extraction Reagent (Millipore) and incubated for 20 min at room temperature as described in the protocol.

    Article Title: Engineering Escherichia coli for increased Und-P availability leads to material improvements in glycan expression technology
    Article Snippet: Total cell material was matched by centrifuging the equivalent of 1 ml of culture at an OD 600 = 1.0 and resuspending the pellet in 25 μL BugBuster® Protein Extraction Reagent (MilliporeSigma) containing 1:100 benzonase.

    Article Title: Engineering Escherichia coli for increased Und-P availability leads to material improvements in glycan expression technology.
    Article Snippet: Total cell material was matched by centrifuging the equivalent of 1 ml of culture at an OD600 = 1.0 and resuspending the pellet in 25 μL BugBuster® Protein Extraction Reagent (MilliporeSigma) containing 1:100 benzonase.

    Sonication:

    Article Title: Identification of functional genes in a chloroform and dichloromethane-degrading microbial culture
    Article Snippet: In a Coy anaerobic glovebox, the pellet was thawed and resuspended in 10 mL anaerobic lysis buffer [50 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.1% Triton-X100, 5% glycerol, 1 mM tris(2-carboxyethyl)phosphine (TCEP), 50 μg/mL leupeptin, 2 μg/mL aprotinin, 10 mM MgCl 2 ], the cells were lysed using BugBuster ® protein extraction reagent (MilliporeSigma), 300 μg/mL lysozyme, and 1 μg/mL DNase.

    Article Title: Method for automated protein purification
    Article Snippet: Other cell lysis methodologies are within the scope of this disclosure, including but not limited to sonication to disrupt cell membranes, chemical lysis (e.g., Bugbuster® Protein Extraction Reagent, Millipore Sigma, Burlington, MA), liquid homogenization (e.g., French Press), mechanical lysis (e.g., rotating blades that grind and disperse cells and tissues), and the like.

    Article Title: Extract2Chip-Bypassing Protein Purification in Drug Discovery Using Surface Plasmon Resonance.
    Article Snippet: The cells were resuspended in BugBuster® Protein Extraction Reagent (Novagen) supplemented with 0.1 mg/mL Lysozyme, 5 U/mL Benzonase, 1 mM PMSF, and PIC without EDTA, then placed on ice for 20 min.

    Article Title: Machine learning reveals signatures of promiscuous microbial amidases for micropollutant biotransformations
    Article Snippet: Induced cells (1 mL volumes) were lysed using the BugBuster® Protein Extraction Reagent (Sigma-Aldrich) according to the manufacturer’s instructions without use of any optional steps.

    Article Title: Supporting Information for Systematic analysis of non-programmed frameshift suppression in E. coli via translational tiling proteomics
    Article Snippet: Cell pellets were resuspended in lysis buffer (BugBuster® Protein Extraction Reagent (MilliporeSigma, MA, USA) supplemented with 1x cOmpleteTM, EDTA-free Protease Inhibitor Cocktail (MilliporeSigma, MA, USA), 1 U/μl rLysozymeTM (MilliporeSigma, MA, USA; final concentration) and 0.5 U/μl Benzonase® Nuclease (MilliporeSigma, MA, USA, final concentration)) resulting in a final OD600 of 20, calculated according to: X μl lysis buffer = OD600 x ml of culture pelleted x 20.

    Article Title: T cell activating bispecific antigen binding molecules
    Article Snippet: The bacterial pellet was resuspended with 25 ml BugBuster® Protein Extraction Reagent (Millipore) and incubated for 20 min at room temperature as described in the protocol.

    Article Title: Engineering Escherichia coli for increased Und-P availability leads to material improvements in glycan expression technology
    Article Snippet: Total cell material was matched by centrifuging the equivalent of 1 ml of culture at an OD 600 = 1.0 and resuspending the pellet in 25 μL BugBuster® Protein Extraction Reagent (MilliporeSigma) containing 1:100 benzonase.

    Article Title: Engineering Escherichia coli for increased Und-P availability leads to material improvements in glycan expression technology.
    Article Snippet: Total cell material was matched by centrifuging the equivalent of 1 ml of culture at an OD600 = 1.0 and resuspending the pellet in 25 μL BugBuster® Protein Extraction Reagent (MilliporeSigma) containing 1:100 benzonase.

    Protein Extraction:

    Article Title: Identification of functional genes in a chloroform and dichloromethane-degrading microbial culture
    Article Snippet: In a Coy anaerobic glovebox, the pellet was thawed and resuspended in 10 mL anaerobic lysis buffer [50 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.1% Triton-X100, 5% glycerol, 1 mM tris(2-carboxyethyl)phosphine (TCEP), 50 μg/mL leupeptin, 2 μg/mL aprotinin, 10 mM MgCl 2 ], the cells were lysed using BugBuster ® protein extraction reagent (MilliporeSigma), 300 μg/mL lysozyme, and 1 μg/mL DNase.

    Article Title: Method for automated protein purification
    Article Snippet: Other cell lysis methodologies are within the scope of this disclosure, including but not limited to sonication to disrupt cell membranes, chemical lysis (e.g., Bugbuster® Protein Extraction Reagent, Millipore Sigma, Burlington, MA), liquid homogenization (e.g., French Press), mechanical lysis (e.g., rotating blades that grind and disperse cells and tissues), and the like.

    Article Title: Extract2Chip-Bypassing Protein Purification in Drug Discovery Using Surface Plasmon Resonance.
    Article Snippet: The cells were resuspended in BugBuster® Protein Extraction Reagent (Novagen) supplemented with 0.1 mg/mL Lysozyme, 5 U/mL Benzonase, 1 mM PMSF, and PIC without EDTA, then placed on ice for 20 min.

    Article Title: Machine learning reveals signatures of promiscuous microbial amidases for micropollutant biotransformations
    Article Snippet: Induced cells (1 mL volumes) were lysed using the BugBuster® Protein Extraction Reagent (Sigma-Aldrich) according to the manufacturer’s instructions without use of any optional steps.

    Article Title: Supporting Information for Systematic analysis of non-programmed frameshift suppression in E. coli via translational tiling proteomics
    Article Snippet: Cell pellets were resuspended in lysis buffer (BugBuster® Protein Extraction Reagent (MilliporeSigma, MA, USA) supplemented with 1x cOmpleteTM, EDTA-free Protease Inhibitor Cocktail (MilliporeSigma, MA, USA), 1 U/μl rLysozymeTM (MilliporeSigma, MA, USA; final concentration) and 0.5 U/μl Benzonase® Nuclease (MilliporeSigma, MA, USA, final concentration)) resulting in a final OD600 of 20, calculated according to: X μl lysis buffer = OD600 x ml of culture pelleted x 20.

    Article Title: T cell activating bispecific antigen binding molecules
    Article Snippet: The bacterial pellet was resuspended with 25 ml BugBuster® Protein Extraction Reagent (Millipore) and incubated for 20 min at room temperature as described in the protocol.

    Article Title: Engineering Escherichia coli for increased Und-P availability leads to material improvements in glycan expression technology
    Article Snippet: Total cell material was matched by centrifuging the equivalent of 1 ml of culture at an OD 600 = 1.0 and resuspending the pellet in 25 μL BugBuster® Protein Extraction Reagent (MilliporeSigma) containing 1:100 benzonase.

    Article Title: Engineering Escherichia coli for increased Und-P availability leads to material improvements in glycan expression technology.
    Article Snippet: Total cell material was matched by centrifuging the equivalent of 1 ml of culture at an OD600 = 1.0 and resuspending the pellet in 25 μL BugBuster® Protein Extraction Reagent (MilliporeSigma) containing 1:100 benzonase.

    Homogenization:

    Article Title: Identification of functional genes in a chloroform and dichloromethane-degrading microbial culture
    Article Snippet: In a Coy anaerobic glovebox, the pellet was thawed and resuspended in 10 mL anaerobic lysis buffer [50 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.1% Triton-X100, 5% glycerol, 1 mM tris(2-carboxyethyl)phosphine (TCEP), 50 μg/mL leupeptin, 2 μg/mL aprotinin, 10 mM MgCl 2 ], the cells were lysed using BugBuster ® protein extraction reagent (MilliporeSigma), 300 μg/mL lysozyme, and 1 μg/mL DNase.

    Article Title: Method for automated protein purification
    Article Snippet: Other cell lysis methodologies are within the scope of this disclosure, including but not limited to sonication to disrupt cell membranes, chemical lysis (e.g., Bugbuster® Protein Extraction Reagent, Millipore Sigma, Burlington, MA), liquid homogenization (e.g., French Press), mechanical lysis (e.g., rotating blades that grind and disperse cells and tissues), and the like.

    Article Title: Extract2Chip-Bypassing Protein Purification in Drug Discovery Using Surface Plasmon Resonance.
    Article Snippet: The cells were resuspended in BugBuster® Protein Extraction Reagent (Novagen) supplemented with 0.1 mg/mL Lysozyme, 5 U/mL Benzonase, 1 mM PMSF, and PIC without EDTA, then placed on ice for 20 min.

    Article Title: Machine learning reveals signatures of promiscuous microbial amidases for micropollutant biotransformations
    Article Snippet: Induced cells (1 mL volumes) were lysed using the BugBuster® Protein Extraction Reagent (Sigma-Aldrich) according to the manufacturer’s instructions without use of any optional steps.

    Article Title: Supporting Information for Systematic analysis of non-programmed frameshift suppression in E. coli via translational tiling proteomics
    Article Snippet: Cell pellets were resuspended in lysis buffer (BugBuster® Protein Extraction Reagent (MilliporeSigma, MA, USA) supplemented with 1x cOmpleteTM, EDTA-free Protease Inhibitor Cocktail (MilliporeSigma, MA, USA), 1 U/μl rLysozymeTM (MilliporeSigma, MA, USA; final concentration) and 0.5 U/μl Benzonase® Nuclease (MilliporeSigma, MA, USA, final concentration)) resulting in a final OD600 of 20, calculated according to: X μl lysis buffer = OD600 x ml of culture pelleted x 20.

    Article Title: T cell activating bispecific antigen binding molecules
    Article Snippet: The bacterial pellet was resuspended with 25 ml BugBuster® Protein Extraction Reagent (Millipore) and incubated for 20 min at room temperature as described in the protocol.

    Article Title: Engineering Escherichia coli for increased Und-P availability leads to material improvements in glycan expression technology
    Article Snippet: Total cell material was matched by centrifuging the equivalent of 1 ml of culture at an OD 600 = 1.0 and resuspending the pellet in 25 μL BugBuster® Protein Extraction Reagent (MilliporeSigma) containing 1:100 benzonase.

    Article Title: Engineering Escherichia coli for increased Und-P availability leads to material improvements in glycan expression technology.
    Article Snippet: Total cell material was matched by centrifuging the equivalent of 1 ml of culture at an OD600 = 1.0 and resuspending the pellet in 25 μL BugBuster® Protein Extraction Reagent (MilliporeSigma) containing 1:100 benzonase.

    Software:

    Article Title: Identification of functional genes in a chloroform and dichloromethane-degrading microbial culture
    Article Snippet: In a Coy anaerobic glovebox, the pellet was thawed and resuspended in 10 mL anaerobic lysis buffer [50 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.1% Triton-X100, 5% glycerol, 1 mM tris(2-carboxyethyl)phosphine (TCEP), 50 μg/mL leupeptin, 2 μg/mL aprotinin, 10 mM MgCl 2 ], the cells were lysed using BugBuster ® protein extraction reagent (MilliporeSigma), 300 μg/mL lysozyme, and 1 μg/mL DNase.

    Article Title: Method for automated protein purification
    Article Snippet: Other cell lysis methodologies are within the scope of this disclosure, including but not limited to sonication to disrupt cell membranes, chemical lysis (e.g., Bugbuster® Protein Extraction Reagent, Millipore Sigma, Burlington, MA), liquid homogenization (e.g., French Press), mechanical lysis (e.g., rotating blades that grind and disperse cells and tissues), and the like.

    Article Title: Extract2Chip-Bypassing Protein Purification in Drug Discovery Using Surface Plasmon Resonance.
    Article Snippet: The cells were resuspended in BugBuster® Protein Extraction Reagent (Novagen) supplemented with 0.1 mg/mL Lysozyme, 5 U/mL Benzonase, 1 mM PMSF, and PIC without EDTA, then placed on ice for 20 min.

    Article Title: Machine learning reveals signatures of promiscuous microbial amidases for micropollutant biotransformations
    Article Snippet: Induced cells (1 mL volumes) were lysed using the BugBuster® Protein Extraction Reagent (Sigma-Aldrich) according to the manufacturer’s instructions without use of any optional steps.

    Article Title: Supporting Information for Systematic analysis of non-programmed frameshift suppression in E. coli via translational tiling proteomics
    Article Snippet: Cell pellets were resuspended in lysis buffer (BugBuster® Protein Extraction Reagent (MilliporeSigma, MA, USA) supplemented with 1x cOmpleteTM, EDTA-free Protease Inhibitor Cocktail (MilliporeSigma, MA, USA), 1 U/μl rLysozymeTM (MilliporeSigma, MA, USA; final concentration) and 0.5 U/μl Benzonase® Nuclease (MilliporeSigma, MA, USA, final concentration)) resulting in a final OD600 of 20, calculated according to: X μl lysis buffer = OD600 x ml of culture pelleted x 20.

    Article Title: T cell activating bispecific antigen binding molecules
    Article Snippet: The bacterial pellet was resuspended with 25 ml BugBuster® Protein Extraction Reagent (Millipore) and incubated for 20 min at room temperature as described in the protocol.

    Article Title: Engineering Escherichia coli for increased Und-P availability leads to material improvements in glycan expression technology
    Article Snippet: Total cell material was matched by centrifuging the equivalent of 1 ml of culture at an OD 600 = 1.0 and resuspending the pellet in 25 μL BugBuster® Protein Extraction Reagent (MilliporeSigma) containing 1:100 benzonase.

    Article Title: Engineering Escherichia coli for increased Und-P availability leads to material improvements in glycan expression technology.
    Article Snippet: Total cell material was matched by centrifuging the equivalent of 1 ml of culture at an OD600 = 1.0 and resuspending the pellet in 25 μL BugBuster® Protein Extraction Reagent (MilliporeSigma) containing 1:100 benzonase.

    Extraction:

    Article Title: Identification of functional genes in a chloroform and dichloromethane-degrading microbial culture
    Article Snippet: In a Coy anaerobic glovebox, the pellet was thawed and resuspended in 10 mL anaerobic lysis buffer [50 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.1% Triton-X100, 5% glycerol, 1 mM tris(2-carboxyethyl)phosphine (TCEP), 50 μg/mL leupeptin, 2 μg/mL aprotinin, 10 mM MgCl 2 ], the cells were lysed using BugBuster ® protein extraction reagent (MilliporeSigma), 300 μg/mL lysozyme, and 1 μg/mL DNase.

    Article Title: Method for automated protein purification
    Article Snippet: Other cell lysis methodologies are within the scope of this disclosure, including but not limited to sonication to disrupt cell membranes, chemical lysis (e.g., Bugbuster® Protein Extraction Reagent, Millipore Sigma, Burlington, MA), liquid homogenization (e.g., French Press), mechanical lysis (e.g., rotating blades that grind and disperse cells and tissues), and the like.

    Article Title: Extract2Chip-Bypassing Protein Purification in Drug Discovery Using Surface Plasmon Resonance.
    Article Snippet: The cells were resuspended in BugBuster® Protein Extraction Reagent (Novagen) supplemented with 0.1 mg/mL Lysozyme, 5 U/mL Benzonase, 1 mM PMSF, and PIC without EDTA, then placed on ice for 20 min.

    Article Title: Machine learning reveals signatures of promiscuous microbial amidases for micropollutant biotransformations
    Article Snippet: Induced cells (1 mL volumes) were lysed using the BugBuster® Protein Extraction Reagent (Sigma-Aldrich) according to the manufacturer’s instructions without use of any optional steps.

    Article Title: Supporting Information for Systematic analysis of non-programmed frameshift suppression in E. coli via translational tiling proteomics
    Article Snippet: Cell pellets were resuspended in lysis buffer (BugBuster® Protein Extraction Reagent (MilliporeSigma, MA, USA) supplemented with 1x cOmpleteTM, EDTA-free Protease Inhibitor Cocktail (MilliporeSigma, MA, USA), 1 U/μl rLysozymeTM (MilliporeSigma, MA, USA; final concentration) and 0.5 U/μl Benzonase® Nuclease (MilliporeSigma, MA, USA, final concentration)) resulting in a final OD600 of 20, calculated according to: X μl lysis buffer = OD600 x ml of culture pelleted x 20.

    Article Title: T cell activating bispecific antigen binding molecules
    Article Snippet: The bacterial pellet was resuspended with 25 ml BugBuster® Protein Extraction Reagent (Millipore) and incubated for 20 min at room temperature as described in the protocol.

    Article Title: Engineering Escherichia coli for increased Und-P availability leads to material improvements in glycan expression technology
    Article Snippet: Total cell material was matched by centrifuging the equivalent of 1 ml of culture at an OD 600 = 1.0 and resuspending the pellet in 25 μL BugBuster® Protein Extraction Reagent (MilliporeSigma) containing 1:100 benzonase.

    Article Title: Engineering Escherichia coli for increased Und-P availability leads to material improvements in glycan expression technology.
    Article Snippet: Total cell material was matched by centrifuging the equivalent of 1 ml of culture at an OD600 = 1.0 and resuspending the pellet in 25 μL BugBuster® Protein Extraction Reagent (MilliporeSigma) containing 1:100 benzonase.

    Protease Inhibitor:

    Article Title: Identification of functional genes in a chloroform and dichloromethane-degrading microbial culture
    Article Snippet: In a Coy anaerobic glovebox, the pellet was thawed and resuspended in 10 mL anaerobic lysis buffer [50 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.1% Triton-X100, 5% glycerol, 1 mM tris(2-carboxyethyl)phosphine (TCEP), 50 μg/mL leupeptin, 2 μg/mL aprotinin, 10 mM MgCl 2 ], the cells were lysed using BugBuster ® protein extraction reagent (MilliporeSigma), 300 μg/mL lysozyme, and 1 μg/mL DNase.

    Article Title: Method for automated protein purification
    Article Snippet: Other cell lysis methodologies are within the scope of this disclosure, including but not limited to sonication to disrupt cell membranes, chemical lysis (e.g., Bugbuster® Protein Extraction Reagent, Millipore Sigma, Burlington, MA), liquid homogenization (e.g., French Press), mechanical lysis (e.g., rotating blades that grind and disperse cells and tissues), and the like.

    Article Title: Extract2Chip-Bypassing Protein Purification in Drug Discovery Using Surface Plasmon Resonance.
    Article Snippet: The cells were resuspended in BugBuster® Protein Extraction Reagent (Novagen) supplemented with 0.1 mg/mL Lysozyme, 5 U/mL Benzonase, 1 mM PMSF, and PIC without EDTA, then placed on ice for 20 min.

    Article Title: Machine learning reveals signatures of promiscuous microbial amidases for micropollutant biotransformations
    Article Snippet: Induced cells (1 mL volumes) were lysed using the BugBuster® Protein Extraction Reagent (Sigma-Aldrich) according to the manufacturer’s instructions without use of any optional steps.

    Article Title: Supporting Information for Systematic analysis of non-programmed frameshift suppression in E. coli via translational tiling proteomics
    Article Snippet: Cell pellets were resuspended in lysis buffer (BugBuster® Protein Extraction Reagent (MilliporeSigma, MA, USA) supplemented with 1x cOmpleteTM, EDTA-free Protease Inhibitor Cocktail (MilliporeSigma, MA, USA), 1 U/μl rLysozymeTM (MilliporeSigma, MA, USA; final concentration) and 0.5 U/μl Benzonase® Nuclease (MilliporeSigma, MA, USA, final concentration)) resulting in a final OD600 of 20, calculated according to: X μl lysis buffer = OD600 x ml of culture pelleted x 20.

    Article Title: T cell activating bispecific antigen binding molecules
    Article Snippet: The bacterial pellet was resuspended with 25 ml BugBuster® Protein Extraction Reagent (Millipore) and incubated for 20 min at room temperature as described in the protocol.

    Article Title: Engineering Escherichia coli for increased Und-P availability leads to material improvements in glycan expression technology
    Article Snippet: Total cell material was matched by centrifuging the equivalent of 1 ml of culture at an OD 600 = 1.0 and resuspending the pellet in 25 μL BugBuster® Protein Extraction Reagent (MilliporeSigma) containing 1:100 benzonase.

    Article Title: Engineering Escherichia coli for increased Und-P availability leads to material improvements in glycan expression technology.
    Article Snippet: Total cell material was matched by centrifuging the equivalent of 1 ml of culture at an OD600 = 1.0 and resuspending the pellet in 25 μL BugBuster® Protein Extraction Reagent (MilliporeSigma) containing 1:100 benzonase.

    Concentration Assay:

    Article Title: Identification of functional genes in a chloroform and dichloromethane-degrading microbial culture
    Article Snippet: In a Coy anaerobic glovebox, the pellet was thawed and resuspended in 10 mL anaerobic lysis buffer [50 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.1% Triton-X100, 5% glycerol, 1 mM tris(2-carboxyethyl)phosphine (TCEP), 50 μg/mL leupeptin, 2 μg/mL aprotinin, 10 mM MgCl 2 ], the cells were lysed using BugBuster ® protein extraction reagent (MilliporeSigma), 300 μg/mL lysozyme, and 1 μg/mL DNase.

    Article Title: Method for automated protein purification
    Article Snippet: Other cell lysis methodologies are within the scope of this disclosure, including but not limited to sonication to disrupt cell membranes, chemical lysis (e.g., Bugbuster® Protein Extraction Reagent, Millipore Sigma, Burlington, MA), liquid homogenization (e.g., French Press), mechanical lysis (e.g., rotating blades that grind and disperse cells and tissues), and the like.

    Article Title: Extract2Chip-Bypassing Protein Purification in Drug Discovery Using Surface Plasmon Resonance.
    Article Snippet: The cells were resuspended in BugBuster® Protein Extraction Reagent (Novagen) supplemented with 0.1 mg/mL Lysozyme, 5 U/mL Benzonase, 1 mM PMSF, and PIC without EDTA, then placed on ice for 20 min.

    Article Title: Machine learning reveals signatures of promiscuous microbial amidases for micropollutant biotransformations
    Article Snippet: Induced cells (1 mL volumes) were lysed using the BugBuster® Protein Extraction Reagent (Sigma-Aldrich) according to the manufacturer’s instructions without use of any optional steps.

    Article Title: Supporting Information for Systematic analysis of non-programmed frameshift suppression in E. coli via translational tiling proteomics
    Article Snippet: Cell pellets were resuspended in lysis buffer (BugBuster® Protein Extraction Reagent (MilliporeSigma, MA, USA) supplemented with 1x cOmpleteTM, EDTA-free Protease Inhibitor Cocktail (MilliporeSigma, MA, USA), 1 U/μl rLysozymeTM (MilliporeSigma, MA, USA; final concentration) and 0.5 U/μl Benzonase® Nuclease (MilliporeSigma, MA, USA, final concentration)) resulting in a final OD600 of 20, calculated according to: X μl lysis buffer = OD600 x ml of culture pelleted x 20.

    Article Title: T cell activating bispecific antigen binding molecules
    Article Snippet: The bacterial pellet was resuspended with 25 ml BugBuster® Protein Extraction Reagent (Millipore) and incubated for 20 min at room temperature as described in the protocol.

    Article Title: Engineering Escherichia coli for increased Und-P availability leads to material improvements in glycan expression technology
    Article Snippet: Total cell material was matched by centrifuging the equivalent of 1 ml of culture at an OD 600 = 1.0 and resuspending the pellet in 25 μL BugBuster® Protein Extraction Reagent (MilliporeSigma) containing 1:100 benzonase.

    Article Title: Engineering Escherichia coli for increased Und-P availability leads to material improvements in glycan expression technology.
    Article Snippet: Total cell material was matched by centrifuging the equivalent of 1 ml of culture at an OD600 = 1.0 and resuspending the pellet in 25 μL BugBuster® Protein Extraction Reagent (MilliporeSigma) containing 1:100 benzonase.

    Incubation:

    Article Title: Identification of functional genes in a chloroform and dichloromethane-degrading microbial culture
    Article Snippet: In a Coy anaerobic glovebox, the pellet was thawed and resuspended in 10 mL anaerobic lysis buffer [50 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.1% Triton-X100, 5% glycerol, 1 mM tris(2-carboxyethyl)phosphine (TCEP), 50 μg/mL leupeptin, 2 μg/mL aprotinin, 10 mM MgCl 2 ], the cells were lysed using BugBuster ® protein extraction reagent (MilliporeSigma), 300 μg/mL lysozyme, and 1 μg/mL DNase.

    Article Title: Method for automated protein purification
    Article Snippet: Other cell lysis methodologies are within the scope of this disclosure, including but not limited to sonication to disrupt cell membranes, chemical lysis (e.g., Bugbuster® Protein Extraction Reagent, Millipore Sigma, Burlington, MA), liquid homogenization (e.g., French Press), mechanical lysis (e.g., rotating blades that grind and disperse cells and tissues), and the like.

    Article Title: Extract2Chip-Bypassing Protein Purification in Drug Discovery Using Surface Plasmon Resonance.
    Article Snippet: The cells were resuspended in BugBuster® Protein Extraction Reagent (Novagen) supplemented with 0.1 mg/mL Lysozyme, 5 U/mL Benzonase, 1 mM PMSF, and PIC without EDTA, then placed on ice for 20 min.

    Article Title: Machine learning reveals signatures of promiscuous microbial amidases for micropollutant biotransformations
    Article Snippet: Induced cells (1 mL volumes) were lysed using the BugBuster® Protein Extraction Reagent (Sigma-Aldrich) according to the manufacturer’s instructions without use of any optional steps.

    Article Title: Supporting Information for Systematic analysis of non-programmed frameshift suppression in E. coli via translational tiling proteomics
    Article Snippet: Cell pellets were resuspended in lysis buffer (BugBuster® Protein Extraction Reagent (MilliporeSigma, MA, USA) supplemented with 1x cOmpleteTM, EDTA-free Protease Inhibitor Cocktail (MilliporeSigma, MA, USA), 1 U/μl rLysozymeTM (MilliporeSigma, MA, USA; final concentration) and 0.5 U/μl Benzonase® Nuclease (MilliporeSigma, MA, USA, final concentration)) resulting in a final OD600 of 20, calculated according to: X μl lysis buffer = OD600 x ml of culture pelleted x 20.

    Article Title: T cell activating bispecific antigen binding molecules
    Article Snippet: The bacterial pellet was resuspended with 25 ml BugBuster® Protein Extraction Reagent (Millipore) and incubated for 20 min at room temperature as described in the protocol.

    Article Title: Engineering Escherichia coli for increased Und-P availability leads to material improvements in glycan expression technology
    Article Snippet: Total cell material was matched by centrifuging the equivalent of 1 ml of culture at an OD 600 = 1.0 and resuspending the pellet in 25 μL BugBuster® Protein Extraction Reagent (MilliporeSigma) containing 1:100 benzonase.

    Article Title: Engineering Escherichia coli for increased Und-P availability leads to material improvements in glycan expression technology.
    Article Snippet: Total cell material was matched by centrifuging the equivalent of 1 ml of culture at an OD600 = 1.0 and resuspending the pellet in 25 μL BugBuster® Protein Extraction Reagent (MilliporeSigma) containing 1:100 benzonase.



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    https://www.bioz.com/product/bugbuster%C2%AE+protein+extraction+reagent/bugbuster+protein+extraction+reagent/pmc12267110-340-8-12
    Average 90 stars, based on 1 article reviews
    bugbuster protein extraction reagent cat. no. 70584-3 - by Bioz Stars, 2026-09
    90/100 stars
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